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Open AccessArticle

Crosstalk between Mast Cells and Lung Fibroblasts Is Modified by Alveolar Extracellular Matrix and Influences Epithelial Migration

1
Unit of Lung Biology, Department of Experimental Medical Sciences, Lund University, 221 84 Lund, Sweden
2
Department of Respiratory Medicine and Allergology, Sk?ne University Hospital, Lund University, 221 85 Lund, Sweden
3
Lund University Bioimaging Centre, Lund University, 221 85 Lund, Sweden
4
Bioscience Department, Respiratory, Inflammation and Autoimmunity, IMED Biotech Unit, AstraZeneca, 431 53 M?lndal, Sweden
5
Department of Cardiothoracic Surgery and Transplant Institute, Sahlgrenska University Hospital, 413 45 Gothenburg, Sweden
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(2), 506; https://doi.org/10.3390/ijms22020506
Received: 23 November 2020 / Revised: 29 December 2020 / Accepted: 4 January 2021 / Published: 6 January 2021
(This article belongs to the Special Issue Mast Cells and Fibrosis )
Mast cells play an important role in asthma, however, the interactions between mast cells, fibroblasts and epithelial cells in idiopathic pulmonary fibrosis (IPF) are less known. The objectives were to investigate the effect of mast cells on fibroblast activity and migration of epithelial cells. Lung fibroblasts from IPF patients and healthy individuals were co-cultured with LAD2 mast cells or stimulated with the proteases tryptase and chymase. Human lung fibroblasts and mast cells were cultured on cell culture plastic plates or decellularized human lung tissue (scaffolds) to create a more physiological milieu by providing an alveolar extracellular matrix. Released mediators were analyzed and evaluated for effects on epithelial cell migration. Tryptase increased vascular endothelial growth factor (VEGF) release from fibroblasts, whereas co-culture with mast cells increased IL-6 and hepatocyte growth factor (HGF). Culture in scaffolds increased the release of VEGF compared to culture on plastic. Migration of epithelial cells was reduced by IL-6, while HGF and conditioned media from scaffold cultures promoted migration. In conclusion, mast cells and tryptase increased fibroblast release of mediators that influenced epithelial migration. These data indicate a role of mast cells and tryptase in the interplay between fibroblasts, epithelial cells and the alveolar extracellular matrix in health and lung disease. View Full-Text
Keywords: lung fibroblasts; mast cells; epithelial cells; extracellular matrix; IL-6; tryptase; vascular endothelial growth factor; hepatocyte growth factor; idiopathic pulmonary fibrosis lung fibroblasts; mast cells; epithelial cells; extracellular matrix; IL-6; tryptase; vascular endothelial growth factor; hepatocyte growth factor; idiopathic pulmonary fibrosis
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    Link: https://zenodo.org/communities/aerotox/
    Description: The data presented in this study will be openly available at https://zenodo.org/communities/aerotox/ at [doi], reference number?
MDPI and ACS Style

Bagher, M.; Rosmark, O.; Elowsson Rendin, L.; Nybom, A.; Wasserstrom, S.; Müller, C.; Zhou, X.-H.; Dellgren, G.; Hallgren, O.; Bjermer, L.; Larsson-Callerfelt, A.-K.; Westergren-Thorsson, G. Crosstalk between Mast Cells and Lung Fibroblasts Is Modified by Alveolar Extracellular Matrix and Influences Epithelial Migration. Int. J. Mol. Sci. 2021, 22, 506.

AMA Style

Bagher M, Rosmark O, Elowsson Rendin L, Nybom A, Wasserstrom S, Müller C, Zhou X-H, Dellgren G, Hallgren O, Bjermer L, Larsson-Callerfelt A-K, Westergren-Thorsson G. Crosstalk between Mast Cells and Lung Fibroblasts Is Modified by Alveolar Extracellular Matrix and Influences Epithelial Migration. International Journal of Molecular Sciences. 2021; 22(2):506.

Chicago/Turabian Style

Bagher, Mariam; Rosmark, Oskar; Elowsson Rendin, Linda; Nybom, Annika; Wasserstrom, Sebastian; Müller, Catharina; Zhou, Xiao-Hong; Dellgren, G?ran; Hallgren, Oskar; Bjermer, Leif; Larsson-Callerfelt, Anna-Karin; Westergren-Thorsson, Gunilla. 2021. "Crosstalk between Mast Cells and Lung Fibroblasts Is Modified by Alveolar Extracellular Matrix and Influences Epithelial Migration" Int. J. Mol. Sci. 22, no. 2: 506.

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